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mouse multiple tissue cdna panel  (TaKaRa)


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    Structured Review

    TaKaRa mouse multiple tissue cdna panel
    RT-PCR analysis <t>of</t> <t>ODC-AS</t> expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned <t>cDNA</t> plasmid; NC: negative control for G3PDH (water).
    Mouse Multiple Tissue Cdna Panel, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+multiple+tissue+cdna+panel/Mouse+MTC+Panel+I/pmc02765418-57-11-17
    Average 95 stars, based on 168 article reviews
    mouse multiple tissue cdna panel - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells"

    Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells

    Journal: Journal of Biomedical Science

    doi: 10.1186/1423-0127-16-92

    RT-PCR analysis of ODC-AS expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned cDNA plasmid; NC: negative control for G3PDH (water).
    Figure Legend Snippet: RT-PCR analysis of ODC-AS expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned cDNA plasmid; NC: negative control for G3PDH (water).

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Amplification, Positive Control, Clone Assay, Plasmid Preparation, Negative Control

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy
    Article Snippet: .. Real-time PCR was performed with a mouse multiple tissue cDNA panel (Clontech) using variant-specific TaqMan probes and primers. ..

    Article Title: Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy
    Article Snippet: .. Real-time PCR was performed with a mouse multiple tissue cDNA panel (Clontech) using variant-specific TaqMan probes and primers. ..

    Article Title: Translational Downregulation of the Noncatalytic Growth Factor Receptor TrkB.T1 by Ischemic Preconditioning of Primary Neurons
    Article Snippet: .. Unamplified cDNA and the Mouse Multiple Tissue cDNA panel (MTCTM , Clontech) served as template in real-time PCR performed on a ABI PRISM 7900HT real-time PCR cycler (Applied Biosystems) using the qPCRTM Core Kit for SybrTM Green I (Eurogentec) according to the manual. ..

    Variant Assay:

    Article Title: Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy
    Article Snippet: .. Real-time PCR was performed with a mouse multiple tissue cDNA panel (Clontech) using variant-specific TaqMan probes and primers. ..

    Article Title: Identification of the Mtus1 Splice Variant as a Novel Inhibitory Factor Against Cardiac Hypertrophy
    Article Snippet: .. Real-time PCR was performed with a mouse multiple tissue cDNA panel (Clontech) using variant-specific TaqMan probes and primers. ..

    Expressing:

    Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells
    Article Snippet: .. Expression of ODC and ODC-AS was analyzed by PCR amplification of mouse Multiple Tissue cDNA panel (MTC, Clontech, Palo Alto, CA) with specific primers (5'ODC: 5'-CCCTGGTCGCGAAGATGTC-3', 5'ODC-AS: 5'-CAGTCGACCTACGTACCATG-3' and common 3' primer: 5'-CGATTGACTTGCAAGCCAAC-3') at the following conditions: 40 cycles of 95°C for 30 seconds, 68°C for 90 seconds with extension at 72°C for 3 minutes using Advantage II PCR kit (Clontech) (Additional file ). ..

    Polymerase Chain Reaction:

    Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells
    Article Snippet: .. Expression of ODC and ODC-AS was analyzed by PCR amplification of mouse Multiple Tissue cDNA panel (MTC, Clontech, Palo Alto, CA) with specific primers (5'ODC: 5'-CCCTGGTCGCGAAGATGTC-3', 5'ODC-AS: 5'-CAGTCGACCTACGTACCATG-3' and common 3' primer: 5'-CGATTGACTTGCAAGCCAAC-3') at the following conditions: 40 cycles of 95°C for 30 seconds, 68°C for 90 seconds with extension at 72°C for 3 minutes using Advantage II PCR kit (Clontech) (Additional file ). ..

    Article Title: Myo1e impairment results in actin reorganization, podocyte dysfunction, and proteinuria in zebrafish and cultured podocytes.
    Article Snippet: .. As a template for PCR analysis, we used cDNA libraries that were generated from various adult mouse tissues (Figure 1C, band 3rd to 10th with lung, brain, testis, spleen, heart, muscle, total kidney, and β-actin) (Mouse Multiple Tissue cDNA Panel I; Clontech Laboratories, Palo Alto, CA). ..

    Article Title: Myo1e Impairment Results in Actin Reorganization, Podocyte Dysfunction, and Proteinuria in Zebrafish and Cultured Podocytes
    Article Snippet: .. As a template for PCR analysis, we used cDNA libraries that were generated from various adult mouse tissues ( , band 3 rd to 10 th with lung, brain, testis, spleen, heart, muscle, total kidney, and β-actin) (Mouse Multiple Tissue cDNA Panel I; Clontech Laboratories, Palo Alto, CA). ..

    Amplification:

    Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells
    Article Snippet: .. Expression of ODC and ODC-AS was analyzed by PCR amplification of mouse Multiple Tissue cDNA panel (MTC, Clontech, Palo Alto, CA) with specific primers (5'ODC: 5'-CCCTGGTCGCGAAGATGTC-3', 5'ODC-AS: 5'-CAGTCGACCTACGTACCATG-3' and common 3' primer: 5'-CGATTGACTTGCAAGCCAAC-3') at the following conditions: 40 cycles of 95°C for 30 seconds, 68°C for 90 seconds with extension at 72°C for 3 minutes using Advantage II PCR kit (Clontech) (Additional file ). ..

    Generated:

    Article Title: Myo1e impairment results in actin reorganization, podocyte dysfunction, and proteinuria in zebrafish and cultured podocytes.
    Article Snippet: .. As a template for PCR analysis, we used cDNA libraries that were generated from various adult mouse tissues (Figure 1C, band 3rd to 10th with lung, brain, testis, spleen, heart, muscle, total kidney, and β-actin) (Mouse Multiple Tissue cDNA Panel I; Clontech Laboratories, Palo Alto, CA). ..

    Article Title: Myo1e Impairment Results in Actin Reorganization, Podocyte Dysfunction, and Proteinuria in Zebrafish and Cultured Podocytes
    Article Snippet: .. As a template for PCR analysis, we used cDNA libraries that were generated from various adult mouse tissues ( , band 3 rd to 10 th with lung, brain, testis, spleen, heart, muscle, total kidney, and β-actin) (Mouse Multiple Tissue cDNA Panel I; Clontech Laboratories, Palo Alto, CA). ..



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    Image Search Results


    RT-PCR analysis of ODC-AS expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned cDNA plasmid; NC: negative control for G3PDH (water).

    Journal: Journal of Biomedical Science

    Article Title: Involvement of an alternatively spliced mitochondrial oxodicarboxylate carrier in adipogenesis in 3T3-L1 cells

    doi: 10.1186/1423-0127-16-92

    Figure Lengend Snippet: RT-PCR analysis of ODC-AS expression in 3T3-L1 cells . Expression of ODC-AS and ODC mRNA in 3T3-L1 Cells were detected with RT-PCR in mRNAs prepared at the indicated days from 3T3-L1 cell pre- and after differentiation, and visualized under UV after agarose gel electrophoresis with ethidium bromide. 1 kb DNA standards is shown on the right. The addition of differentiation reagents was indicated at the bottom. G3PDH was amplified as an internal control and shown below. L: 1 kb DNA ladder; PC: positive control for ODC from cloned cDNA plasmid; NC: negative control for G3PDH (water).

    Article Snippet: Expression of ODC and ODC-AS was analyzed by PCR amplification of mouse Multiple Tissue cDNA panel (MTC, Clontech, Palo Alto, CA) with specific primers (5'ODC: 5'-CCCTGGTCGCGAAGATGTC-3', 5'ODC-AS: 5'-CAGTCGACCTACGTACCATG-3' and common 3' primer: 5'-CGATTGACTTGCAAGCCAAC-3') at the following conditions: 40 cycles of 95°C for 30 seconds, 68°C for 90 seconds with extension at 72°C for 3 minutes using Advantage II PCR kit (Clontech) (Additional file ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Agarose Gel Electrophoresis, Amplification, Positive Control, Clone Assay, Plasmid Preparation, Negative Control